C-Reactive Protein (CRP) Test- Principle, Uses, Procedure ...

C-Reactive Protein (CRP), also known as Pentraxin 1, is a non-glycosylated protein in the Pentraxin family that also includes Pentraxin 2/SAP and Pentraxin 3/TSG-14. CRP is an acute phase reactant, a protein made by the liver and released into the blood within a few hours after tissue injury, the start of an infection, or other cause of inflammation.

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A high level of CRP in the blood is a sign that there may be an inflammatory process occurring in the body. Inflammation itself isn&#;t typically a problem, but it can indicate a host of other health concerns, including infection, arthritis, kidney failure, and pancreatitis. High CRP levels may put patients at increased risk for coronary artery disease, which can cause a heart attack.

A CRP test is a blood test designed to measure the amount of CRP in the blood.

Principle of CRP Test

The C-Reactive Protein test is based on the principle of the latex agglutination. When latex particles complexed human anti-CRP are mixed with a patient&#;s serum containing C reactive proteins, an visible agglutination reaction will take place within 2 minutes.

Uses of CRP Test

1. CRP may be used to detect or monitor significant inflammation in an individual who is suspected of having an acute condition, such as serious bacterial infection like sepsis, a fungal infection and Pelvic inflammatory disease (PID).
2. The CRP test is useful in monitoring people with chronic inflammatory conditions to detect flare-ups and/or to determine if treatment is effective. Some examples include Inflammatory bowel disease, some forms of arthritis and Autoimmune diseases, such as lupus or vasculitis.
3. The determination of the CRP-level is useful to monitor the therapy.
4. It is done to check for infection after surgery. CRP levels normally rise within 2 to 6 hours of surgery and then go down by the third day after surgery. If CRP levels stay elevated 3 days after surgery, an infection may be present.

Procedure of CRP Test

Qualitative Test

1. Bring all reagents and serum sample to Room Temperature and mix latex reagent gently prior to use. Do not dilute the controls and serum.
2. Place 1 drop of Serum, Positive control and Negative control on separate reaction circle on glass slide.
3. Then add 1 drop of CRP latex reagent to each of the circles.
4. Mix with separate mixing sticks and spread the fluid over the entire area of the cell.
5. Tilt the slide back and forth slowly for 2 minutes observing preferably under artificial light.
6. Observe for visible agglutination.

Semi-Quantitative Test

1. Prepare dilution of the specimen with physiological saline 0.9%, as indicated in the following table
2. Then proceed for each dilution as in qualitative test.

Result Interpretation of CRP Test

Positive: Agglutination of latex particles, indicating the presence of C &#; reactive protein at a significant and detectable level.

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Negative: No Agglutination.

For Semi-Quantitative Test Results, the last dilution of serum with visible agglutination is the CRP titre of the serum. 

CALCULATION OF TITRE:

CRP ug/ml = 7 x D, where D is the highest dilution of serum showing agglutination and 7 is the sensitivity in ug/ml.

Limitations of CRP Test

1. The strength of the agglutination reaction is not indicative of the CRP concentration. Weak reactions may occur with slightly elevated or markedly elevated concentrations.
2. A prozone phenomena (antigen excess) may cause false negatives. It is advisable, therefore, to check all negative sera by retesting at a 1:10 dilution.
3. Reaction times longer than specified may produce apparent false reactions due to a drying effect.
4. Strongly lipemic or contaminated sera can cause false positive reactions.
5. Only serum should be used in this test.
6. A quantitative titration procedure on positive specimens is required to observe increasing or
   decreasing levels.
7. Patients with high titers of rheumatoid factors may give positive results.

References

1. Healthline- C-Reactive Protein Test.
2. R&D Systems, Quantikine ELISA- Human C-Reactive Protein/CRP Immunoassay.
3. Swemed Diagnostics- CRP Test.
4. Genix Technology- RapidTex CRP Latex Test.
5. University of Washington Medical Center- C-Reactive Protein.
6. MLAB Immunology/Serology- Laboratory 7: C-Reactive Protein Testing.
7. Lab Tests Online- C-Reactive Protein.
8. Mayo Clinic- Tests and Procedures- C-reactive protein test.
9. MedlinePlus- C-reactive protein.
10. MedicineNet- C-Reactive Protein (CRP).
11. eMedicineHealth- C-Reactive Protein Test (CRP).
12. Life Extension- Testing For C-reactive Protein May Save Your Life.
13. KidsHealth, Medical Tests & Exams, Blood Test: C-Reactive Protein (CRP).
14. WebMD- Digestive health centre- C-reactive protein (CRP).
15. WebMD- Heart Disease and C-Reactive Protein (CRP) Testing.
16. Share Care- What do C-reactive protein (CRP) test levels indicate?
17. Walk-In-Lab, C-Reactive Protein (CRP) Blood Test, Quantitative.
18. Australian Prescriber- C-reactive protein.
19. The New York Times, Health Guide- C-Reactive Protein.
20. About- What Is the C-Reactive Protein (CRP) Test?
21. Group Health- C-Reactive Protein (CRP).
22. Health Link BC, Medical Tests A-Z, C-Reactive Protein (CRP).
23. Diagnostic Automation/Cortez Diagnostics Inc.- CRP Latex Test Kit (Serology test).
24. Laboratory Info
25. Wikipedia

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C-Reactive Protein (CRP) Serum Whole Blood Rapid Test description:

"Export Use Only"
Diagnostic Automation offers a C-Reactive Protein (CRP) Serum/Whole Blood Rapid Test (Cassette) RapiCard Insta Test which is a one-step immunochromatography based test for diagnostic. This CRP Serum/Whole Blood Rapid Test is designed for semi-quantitative determination of CRP in human serum or whole blood specimens. The sensitivity of the CRP Rapid Test can semi-quantitatively detect CRP in serum or whole blood at: < 5 ug / mL, 5-10 ug/mL and > 10 ug/mL ranges.






Materials Provided with CRP Rapid Test kit:
1. CRP Rapid Test device
2. Sample buffer

Materials required but not provided:
1. Serum collection containers.
2. Timer or Clock




CRP Rapid Test kit Background Information
CPR is composed of five protein subunits, produced by the liver and found in blood that plays a key role in the innate immune response. Strong evidence indicates that patients with elevated basal levels of CRP are at an increased risk of hypertension, heart diseases, and diabetes. CRP is proposed as a screening test for predicting risk and guiding preventive approaches in cardiac diseases Normal concentration in healthy human serum is usually lower than 10 mg/L, slightly increasing with aging. Higher levels are found in late pregnant women, mild inflammation and viral infections (10-40 mg/L), active inflammation, bacterial infection (40-200 mg/L), severe bacterial infections and burns (>200 mg/L). The half-life of CRP is constant, thus, CRP level is mainly determined by the rate of its production. A level above 2.4 mg/L has been associated with a doubled risk of a coronary event compared to levels below 1 mg/L. Inflammation can play an important role in atherosclerosis, the process in which fatty deposits build up in coronary arteries. Interest in CRP originated when studies found that patients with unstable angina or chest pain had high levels of this marker. Researchers found that CRP could be used to predict who would go on to have a heart attack. Moreover, in a meta-analysis of 20 studies involving 1,466 patients with coronary artery disease, CRP levels were found to be reduced after exercise interventions. Among those studies, higher CRP concentrations or poorer lipid profiles before beginning exercise were associated with greater reductions in CRP. Other studies have shown that CRP is not a predictor of heart attack risk in people without symptoms of heart disease.






CRP Rapid Test Principle
This CRP Serum/Whole Blood Rapid Test is based on the principle of specific immunochemical reaction between antibodies and antigens to analyze particular compounds in human serum specimen. The CRP Rapid Test relies on the competition for binding antibody between CRP-dye conjugate and free CRP which may be present in the specimen being tested. Specimen collection and preparation instructions are easy to follow. The CRP Rapid Test is safe, accurate, and quick. The range of CRP concentration in serum or whole blood can be detected in 10 minutes. See product insert for more details.



Diagnostic Automation Inc. offers an additional CRP Rapid Test - the CRP Serum Rapid Test. Other Cardiac Markers Rapid Tests are available, along with Cardiac Markers ELISA Tests. Please see our home page product list, or contact our Customer Service Representatives for more information.



For a complete list of Rapid Tests, ELISA tests, IFA Kits, Serology Tests, and available testing Instrumentation, please visit our website home page or contact our Customer Service Representatives at 818-591-.




*Not to be sold, shipped, or distributed in the United States.

"Export Use Only"Diagnostic Automation offers a C-Reactive Protein (CRP) Serum/Whole Blood Rapid Test (Cassette) RapiCard Insta Test which is a one-step immunochromatography based test for diagnostic. This CRP Serum/Whole Blood Rapid Test is designed for semi-quantitative determination of CRP in human serum or whole blood specimens. The sensitivity of the CRP Rapid Test can semi-quantitatively detect CRP in serum or whole blood at: < 5 ug / mL, 5-10 ug/mL and > 10 ug/mL ranges.1. CRP Rapid Test device2. Sample buffer1. Serum collection containers.2. Timer or ClockCPR is composed of five protein subunits, produced by the liver and found in blood that plays a key role in the innate immune response. Strong evidence indicates that patients with elevated basal levels of CRP are at an increased risk of hypertension, heart diseases, and diabetes. CRP is proposed as a screening test for predicting risk and guiding preventive approaches in cardiac diseases Normal concentration in healthy human serum is usually lower than 10 mg/L, slightly increasing with aging. Higher levels are found in late pregnant women, mild inflammation and viral infections (10-40 mg/L), active inflammation, bacterial infection (40-200 mg/L), severe bacterial infections and burns (>200 mg/L). The half-life of CRP is constant, thus, CRP level is mainly determined by the rate of its production. A level above 2.4 mg/L has been associated with a doubled risk of a coronary event compared to levels below 1 mg/L. Inflammation can play an important role in atherosclerosis, the process in which fatty deposits build up in coronary arteries. Interest in CRP originated when studies found that patients with unstable angina or chest pain had high levels of this marker. Researchers found that CRP could be used to predict who would go on to have a heart attack. Moreover, in a meta-analysis of 20 studies involving 1,466 patients with coronary artery disease, CRP levels were found to be reduced after exercise interventions. Among those studies, higher CRP concentrations or poorer lipid profiles before beginning exercise were associated with greater reductions in CRP. Other studies have shown that CRP is not a predictor of heart attack risk in people without symptoms of heart disease.This CRP Serum/Whole Blood Rapid Test is based on the principle of specific immunochemical reaction between antibodies and antigens to analyze particular compounds in human serum specimen. The CRP Rapid Test relies on the competition for binding antibody between CRP-dye conjugate and free CRP which may be present in the specimen being tested. Specimen collection and preparation instructions are easy to follow. The CRP Rapid Test is safe, accurate, and quick. The range of CRP concentration in serum or whole blood can be detected in 10 minutes. See product insert for more details.Diagnostic Automation Inc. offers an additional CRP Rapid Test - the CRP Serum Rapid Test. Other Cardiac Markers Rapid Tests are available, along with Cardiac Markers ELISA Tests. Please see our home page product list, or contact our Customer Service Representatives for more information.For a complete list of Rapid Tests, ELISA tests, IFA Kits, Serology Tests, and available testing Instrumentation, please visit our website home page or contact our Customer Service Representatives at 818-591-.*Not to be sold, shipped, or distributed in the United States.

Contact us to discuss your requirements of Rapid Fob Test Strip. Our experienced sales team can help you identify the options that best suit your needs.